Journal: Journal of Neuropathology and Experimental Neurology
Article Title: Absence of Alzheimer Disease Neuropathologic Changes in Eyes of Subjects With Alzheimer Disease
doi: 10.1093/jnen/nlx020
Figure Lengend Snippet: Description of Cases, Tissue Processing, Methods, Specific Stains Used, and Results in Each Publication That Examined Human Eyes for AD Related Neuropathological Changes
Article Snippet: Koronyo-Hamaoui et al ( 17 ) , 8 patients with definite AD, 5 patients with possible or probable AD, and 5 age-matched controls (autopsy confirmed) , 14 eyes fixed in 10% buffered formalin, retinas dissected free, and whole mounts prepared 4 eyes snap frozen, hyaluronidase treated, fixed in 2.5% paraformaldehyde, retinas dissected free, and whole mounts prepared , IHC 2 , IF 3 : Monoclonal mouse anti-human Aβ against human amino acid (aa) residues 1-16 [clone 6E10 (Millipore, Temecula, CA) and DE2b4 (AbD Serotec, Raleigh NC)], aa 17-24 (clone 4G8, Covance, Princeton, NJ), aa 34-40 (clone 11A5-B10, Millipore), and aa 36-42 (clone 12F4, Covance) IP 4 : clone 12F4 , Positive for β-amyloid in AD patients’ retinas by IF and IP staining (negative in 5 age matched controls).
Techniques: Staining, Formalin-fixed Paraffin-Embedded, Phospho-proteomics, Diagnostic Assay, Saline, Enzyme-linked Immunosorbent Assay, Immunohistochemistry